pUC19

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The gene sequences divided into three successively inserted into a plasmid vector , PUC19 / 18 .
将其全基因序列分三段先后插入到PUC19/18质粒载体中。
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In order to connect with PUC19 vector , the gene fragments of heat shock protein HSP70 were linked with the double-stranded artificial joints With EcoR I restriction site .
以拟南芥为材料,对热激蛋白HSP70基因片段进行了克隆研究,获得了最佳的分子克隆实验体系。